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2-Vessel Occlusion/Hypotension: A Rat Model of Global Brain Ischemia
Published on: June 22, 2013
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Protocol for establishing a global ischemia model using a 4-vessel occlusion in rats
Hyunha Kim1, Rachael Urquhart1, Fabrizio Pontarelli2
1Department of Pharmacology and Neuroscience, Creighton University School of Medicine, Omaha, NE 68178, USA.
STAR Protocols
|January 24, 2024
Summary
This study details a rat model for global cerebral ischemia, a condition blocking blood flow to the brain. The protocol helps researchers investigate neurodegeneration and cognitive deficits following such events.
Area of Science:
- Neuroscience
- Pathology
Background:
- Global cerebral ischemia involves temporary blockage of blood flow to the entire brain.
- This condition can lead to delayed neurological deficits and neurodegeneration.
Purpose of the Study:
- To present a detailed protocol for the four-vessel occlusion rat model.
- To facilitate the study of neurodegeneration and cognitive deficits in global ischemia.
Main Methods:
- The protocol involves performing four-vessel occlusion in rats.
- Specific steps for vertebral and common carotid artery occlusion are described to ensure adequate cerebral blood flow blockage.
Main Results:
- The study outlines expected outcomes observable through histology assays.
- Behavioral tests are detailed to assess cognitive deficits.
Conclusions:
- The four-vessel occlusion rat model provides a method to study global cerebral ischemia.
- This model aids in understanding the neurodegenerative and cognitive consequences of interrupted brain blood flow.
