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θ-Nanopore Ratiometry
Hai-Yan Wang1, Bing Wang1, Chao Sun1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
ACS Nano
|January 24, 2024
Summary
We developed a new nanoscale ratiometric technique called θ-nanopore ratiometry. This method overcomes device variability and nonspecific adsorption for precise biochemical detection in single-cell studies.
Area of Science:
- Nanotechnology
- Biochemistry
- Analytical Chemistry
Background:
- Developing nanoscale techniques for biochemical analysis is crucial for applications with strict spatial and biological constraints.
- Existing methods face challenges with device fabrication variability and nonspecific adsorption, limiting detection accuracy.
Purpose of the Study:
- To introduce and demonstrate the concept of θ-nanopore ratiometry, a novel nanoscale ratiometric technique.
- To address and mitigate concerns regarding device deviation and nonspecific adsorption in nanoscale detection.
Main Methods:
- Development of the θ-nanopore ratiometry concept.
- Fabrication of a 200 nm θ-nanopore device.
- Application of the technique for microRNA (miRNA) detection in a complex cytosolic environment.
Main Results:
- The θ-nanopore ratiometry effectively mitigated ±20 nm aperture drift.
- Nonspecific adsorption issues were significantly minimized in the cytosolic environment.
- Successful measurement of cytosolic miRNA-10b was achieved using this technique.
Conclusions:
- θ-nanopore ratiometry establishes a robust nanoscopic ratiometric technique.
- This method enhances the toolkit for single-cell studies and precise biochemical analysis.
- The technique offers improved accuracy and reliability for nanoscale detection in complex biological samples.
