Circulating microRNA profiling identifies microRNAs linked to prediabetes associated with alcohol dependence syndrome

Area of Science:

• Biochemistry
• Genetics
• Endocrinology

Background:

• MicroRNAs are key gene regulators found in serum, linked to various diseases.
• Alcohol dependence syndrome (ADS) and prediabetes represent significant health concerns.

Purpose of the Study:

• To identify and confirm serum microRNAs (miRNAs) associated with prediabetes in individuals with alcohol dependence syndrome (ADS).

Main Methods:

• Microarray analysis of serum samples from ADS patients (with/without prediabetes) and controls.
• Validation of candidate miRNAs using reverse transcription quantitative polymerase chain reaction (RT-qPCR).
• Gene Ontology (GO) and KEGG pathway analyses to predict miRNA target genes.

Main Results:

• Significant differential expression of 198 and 172 miRNAs in ADS patients with/without prediabetes versus controls.
• Seven miRNAs were uniquely altered in prediabetic ADS patients compared to non-diabetic ADS patients.
• Serum hsa-miR-320b and hsa-miR-3135b were exclusively differentially expressed in prediabetic ADS patients and validated.
• Pathway analysis indicated enrichment of diabetes-related biological processes and pathways.

Conclusions:

• Individuals with ADS, with or without prediabetes, exhibit distinct serum miRNA profiles compared to healthy individuals.
• Serum hsa-miR-320b and hsa-miR-3135b show promise as diagnostic biomarkers for prediabetes in the context of ADS.