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Updated: Jul 30, 2026
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A Decentralized Ex Vivo Murine Bladder Model with the Detrusor Muscle Removed for Direct Access to the Suburothelium during Bladder Filling
Published on: November 28, 2019
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Metabolomic profiling of human bladder tissue extracts.
Krzysztof Ossoliński1, Tomasz Ruman2, Valérie Copié3
1Department of Urology, John Paul II Hospital, Grunwaldzka 4 St., 36-100, Kolbuszowa, Poland.
Metabolomics : Official Journal of the Metabolomic Society
|January 24, 2024
Summary
This study identified key metabolite markers in bladder cancer tissues using NMR and LDI-MS. These findings offer potential for improved bladder cancer detection and monitoring.
Area of Science:
- Biochemistry
- Oncology
- Analytical Chemistry
Background:
- Bladder cancer is a prevalent malignancy with a need for effective biomarkers.
- Current methods for monitoring therapeutic interventions in bladder cancer are insufficient.
Purpose of the Study:
- To conduct metabolomic profiling of human bladder cancer and adjacent normal tissues.
- To identify and evaluate potential cancer biomarkers for bladder cancer detection and monitoring.
Main Methods:
- Utilized nuclear magnetic resonance (NMR) spectroscopy and high-resolution nanoparticle-based laser desorption/ionization mass spectrometry (LDI-MS).
- Analyzed polar metabolite profiles in tissue samples from 99 bladder cancer patients.
Main Results:
- NMR identified six potential bladder cancer tissue metabolites.
- LDI-MS detected 34 compounds distinguishing cancer from normal tissue.
- Metabolites correlated with tumor grades (13) and stages (13); AUC values > 0.853.
Conclusions:
- This is the first study to analyze adjacent human bladder tissues using both NMR and MS.
- Identified metabolite markers show promise for detecting and monitoring bladder cancer stages and grades.
