Determination of bromide ion concentration in greyhound urine by ion chromatography

An ion chromatographic method was used to determine Br ion in the urine of Greyhounds. Phenol was added to the urine, before filtration through a 30,000-molecular weight cutoff filter and to the eluant (pH 11, NaOH-Na2CO3), as a preservative. Urine from 103 racing Greyhounds resulted in a mean +/- SD of 3.60 +/- 2.72 mg of Br/L of urine. Urine samples obtained from a Greyhound, to which 2 g of KBr was administered, yielded a diurnal elimination pattern with a half-life of 7.6 days.