Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

2.2K
Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
2.2K
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

765
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
765
Atomic Absorption Spectroscopy: Radiation and Light Sources01:13

Atomic Absorption Spectroscopy: Radiation and Light Sources

399
Atomic absorption spectroscopy (AAS) relies on the Beer-Lambert law, which requires that the radiation source emits a narrow range of wavelengths to match the absorption characteristics of the analyte atom. The primary criteria for choosing an appropriate radiation source in AAS is to provide a precise and intense emission at specific wavelengths that will allow accurate detection of the analyte.
Two common narrow-range 'line' sources used in AAS are hollow-cathode lamps (HCLs) and...
399
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

163
AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
163
Atomic Absorption Spectroscopy: Overview01:27

Atomic Absorption Spectroscopy: Overview

2.1K
Atomic absorption spectroscopy (AAS) is a technique used to analyze elements by measuring electromagnetic radiation (EMR) absorbed by atoms, which causes them to transition to a higher-energy orbit. The most crucial step in AAS is atomization, where the analyte is converted into gas-phase atoms, typically through a flame or furnace. Some of these atoms become thermally excited in the flame, while most remain in the ground state.
When irradiated by EMR of a particular wavelength, these...
2.1K
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

977
Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
977

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optical and Electron Transparent Polycrystalline Boron Doped Diamond Membranes for Nanoscale Correlative Structure-Electrochemical Measurements.

ACS nano·2025
Same author

Ferroelastic writing of crystal directions in oxide thin films.

Nature nanotechnology·2025
Same author

Toward a Procedure for the Template Free Growth of Te Nanowires Across an Insulator by Electrodeposition.

The journal of physical chemistry. C, Nanomaterials and interfaces·2024
Same author

Superstructure reflections in tilted perovskites.

Acta crystallographica. Section A, Foundations and advances·2024
Same author

An ultra high-endurance memristor using back-end-of-line amorphous SiC.

Scientific reports·2024
Same author

3D electron diffraction goes multipolar.

IUCrJ·2024
Same journal

Case study of using the single-atom R1 method to solve a small protein structure.

Acta crystallographica. Section A, Foundations and advances·2026
Same journal

Beyond complementarity: a reverse-engineering framework for de novo crystal structure determination from EXAFS.

Acta crystallographica. Section A, Foundations and advances·2026
Same journal

Crystallography in Open Science and its open educational resources.

Acta crystallographica. Section A, Foundations and advances·2026
Same journal

From atoms to a data bank: optimizing transferability of electron-density symmetry.

Acta crystallographica. Section A, Foundations and advances·2026
Same journal

Twenty-Sixth General Assembly and International Congress of Crystallography, Melbourne, Australia, 22-29 August 2023.

Acta crystallographica. Section A, Foundations and advances·2026
Same journal

MIDAS: a quantitative framework for high-energy diffraction microscopy. Part II: accuracy, robustness and best practices.

Acta crystallographica. Section A, Foundations and advances·2026
See all related articles

Related Experiment Video

Updated: Jul 5, 2025

Scattering And Absorption of Light in Planetary Regoliths
11:34

Scattering And Absorption of Light in Planetary Regoliths

Published on: July 1, 2019

10.3K

Parameterized absorptive electron scattering factors.

M Thomas1, A Cleverley2, R Beanland1

  • 1Department of Physics, University of Warwick, Coventry CV4 7AL, UK.

Acta Crystallographica. Section A, Foundations and Advances
|January 25, 2024
PubMed
Summary
This summary is machine-generated.

This study simplifies diffuse scattering calculations in electron diffraction by parameterizing the imaginary scattering factor (f') for 103 elements. This accelerates structure solution and refinement methods in materials science.

Keywords:
3D-EDabsorptionelectron diffractionthermal diffuse scatteringthree-dimensional electron diffraction

More Related Videos

In situ Grazing Incidence Small Angle X-ray Scattering on Roll-To-Roll Coating of Organic Solar Cells with Laboratory X-ray Instrumentation
06:49

In situ Grazing Incidence Small Angle X-ray Scattering on Roll-To-Roll Coating of Organic Solar Cells with Laboratory X-ray Instrumentation

Published on: March 2, 2021

6.3K
X-ray Beam Induced Current Measurements for Multi-Modal X-ray Microscopy of Solar Cells
00:10

X-ray Beam Induced Current Measurements for Multi-Modal X-ray Microscopy of Solar Cells

Published on: August 20, 2019

13.8K

Related Experiment Videos

Last Updated: Jul 5, 2025

Scattering And Absorption of Light in Planetary Regoliths
11:34

Scattering And Absorption of Light in Planetary Regoliths

Published on: July 1, 2019

10.3K
In situ Grazing Incidence Small Angle X-ray Scattering on Roll-To-Roll Coating of Organic Solar Cells with Laboratory X-ray Instrumentation
06:49

In situ Grazing Incidence Small Angle X-ray Scattering on Roll-To-Roll Coating of Organic Solar Cells with Laboratory X-ray Instrumentation

Published on: March 2, 2021

6.3K
X-ray Beam Induced Current Measurements for Multi-Modal X-ray Microscopy of Solar Cells
00:10

X-ray Beam Induced Current Measurements for Multi-Modal X-ray Microscopy of Solar Cells

Published on: August 20, 2019

13.8K

Area of Science:

  • Materials Science
  • Crystallography
  • Condensed Matter Physics

Background:

  • Thermal atomic motion in electron diffraction causes diffuse scattering, appearing as a background that obscures Bragg spot intensities.
  • This diffuse scatter is typically subtracted in structure solution methods, complicating data analysis.
  • Modeling diffuse scatter is crucial for accurate interpretation of electron diffraction patterns.

Purpose of the Study:

  • To develop a computationally efficient method for modeling diffuse scattering in electron diffraction.
  • To provide a parameterized form of the imaginary scattering factor (f") for accelerated calculations.
  • To validate the applicability of a simplified two-beam Einstein model for structure solution and refinement.

Main Methods:

  • Utilized the Bloch wave methodology to model electron flux transfer from Bragg spots to diffuse scatter using complex scattering factors (f + if").
  • Employed a two-beam Einstein model to derive the imaginary scattering factor (f").
  • Developed a parameterized form of f" for 103 elements, assuming neutral, spherical atoms.

Main Results:

  • A parameterized form of f" was successfully generated for 103 elements.
  • The proposed method significantly reduces calculation time for diffuse scatter simulations.
  • The simplified two-beam model is deemed appropriate for current structure solution and refinement techniques.

Conclusions:

  • The parameterized imaginary scattering factor offers a substantial computational advantage for electron diffraction studies.
  • This approach enhances the efficiency of materials structure determination using electron diffraction.
  • The study advocates for the use of simplified diffuse scatter models in practical structure solution workflows.