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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Jul 5, 2025

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current

Published on: November 3, 2020

Electrophysiological Methods to Measure Ca²⁺ Current.

Shuang Liu¹

¹Department of Pharmacology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. liussmzk@m.ehime-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)

|January 25, 2024

Summary

This study details the patch-clamp technique for accurately measuring calcium release-activated calcium (CRAC) currents in human T cells. It provides a standard protocol for assessing these crucial ion channel functions.

Keywords:

CRAC-like current Calcium release-activated calcium channel Patch clamp Store-operated Ca2+ entry T cell

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Last Updated: Jul 5, 2025

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current

Published on: November 3, 2020

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents

Published on: July 3, 2013

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Published on: December 8, 2013

Area of Science:

Immunology
Cell Physiology
Molecular Biology

Background:

Accurate assessment of ion channel function is critical for understanding cellular processes.
Store-operated calcium entry (SOCE) plays a vital role in immune cell activation.
Calcium release-activated calcium (CRAC) channels are a key component of SOCE.

Purpose of the Study:

To introduce a standard protocol for detecting calcium release-activated calcium (CRAC) currents.
To provide a method for assessing functional calcium channel properties in human primary T cells.
To outline the application of the patch-clamp technique for studying CRAC currents.

Main Methods:

Utilizing the patch-clamp technique to record membrane currents.
Focusing on the detection of calcium release-activated calcium (CRAC) currents.
Applying the method to human primary T cells.

Main Results:

The patch-clamp technique enables accurate measurement of CRAC currents.
The described protocol facilitates the study of native channel activity.
This method is suitable for investigating store-operated calcium entry pathways.

Conclusions:

The patch-clamp technique is essential for precise characterization of calcium channel function.
The presented protocol offers a reliable method for studying CRAC currents in T cells.
This approach supports research into the regulation of calcium signaling in immune cells.