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Silk Film Culture System for in vitro Analysis and Biomaterial Design
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Reusable Colorimetric Biosensors on Sustainable Silk-Based Platforms.

Augusto Márquez1, Sara Santiago1,2, Molíria Vieira Dos Santos3

  • 1Instituto de Microelectrónica de Barcelona (IMB-CNM, CSIC), Bellaterra, Barcelona 08193, Spain.

ACS Applied Bio Materials
|January 25, 2024
PubMed
Summary
This summary is machine-generated.

This study presents a novel silk-based biosensor for glucose detection, ideal for resource-limited settings. The reusable biosensor visually indicates glucose levels through color change, requiring no external equipment.

Keywords:
colorimetric biosensordithienylethene mediators, photopatterningglucose detectionphotoelectrochromicpoint of caresilk fibroin

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Area of Science:

  • Biomaterials Science
  • Biosensor Technology
  • Analytical Chemistry

Background:

  • Silk fibroin offers a transparent, flexible, and stable substrate for biosensor development.
  • Existing biosensors often require external instrumentation, limiting their use in resource-limited settings.
  • Developing point-of-care diagnostics with visual readouts is crucial for accessible healthcare.

Purpose of the Study:

  • To develop a novel, instrument-free, point-of-care biosensor for glucose detection using silk fibroin.
  • To utilize photoelectrochromic dithienylethene molecules within a silk film for visual glucose quantification.
  • To demonstrate the biosensor's reusability and potential for detecting other analytes.

Main Methods:

  • Silk fibroin films were doped with glucose oxidase, peroxidase, and dithienylethene photoelectrochromic molecules.
  • Photopatterning via dithienylethene photoisomerization created dose-dependent color changes.
  • Enzymatic reactions in the presence of glucose triggered color disappearance proportional to glucose concentration.

Main Results:

  • The silk-based biosensor visually indicated glucose levels (hypoglycemic, normoglycemic, hyperglycemic) through color intensity changes.
  • Color disappearance was proportional to glucose concentration, enabling visual assessment.
  • The biosensor demonstrated reusability for up to five cycles after UV regeneration.

Conclusions:

  • Silk fibroin is a suitable substrate for creating visual, reusable biosensors for resource-limited environments.
  • The photopatterning approach with photoelectrochromic mediators offers a novel method for biosensor fabrication.
  • This technology can be adapted for detecting other analytes by coupling with different oxidoreductases.