Novel MoS₂-decorated Cu₂O hybrid nanoparticles for enhanced non-enzymatic electrochemical cholesterol detection

Precise identification of cholesterol levels is crucial for the early diagnosis of cardiovascular risk factors. This paper presents a novel approach for cholesterol detection that circumvents the reliance on enzymatic processes. Leveraging the unique properties of advanced materials and electrochemical principles, our non-enzymatic approach demonstrates enhanced sensitivity, specificity, and limit of detection in cholesterol analysis. A non-enzymatic electrochemical biosensor for Cholesterol, employing a nanohybrid comprising Cu₂O nanoparticles decorated with MoS₂, is presented. The cyclic voltammetry (CV), differential pulse voltammetry (DPV), and amperometry techniques were employed to investigate the electrochemical behaviour of the glassy carbon electrode modified with the Cu₂O/MoS₂nanohybrid. The modified electrode exhibited an excellent sensitivity of 111.74μAμM^-1cm^-2through the CV method and showcased a low detection limit of 2.18μM and an expansive linear range spanning 0.1-180μM when employing the DPV method. The electrode also showed good selectivity to various interfering components in 0.1 M NaOH and a satisfied stability of about 15 days at room temperature. The study demonstrates the potential for broader applications in clinical diagnostics and monitoring cardiovascular health, paving the way for a paradigm shift in cholesterol detection methodologies and offering a more efficient and cost-effective alternative to traditional enzymatic assays.