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Induced parasexual processes in Claviceps sp. strain SD58.

K L Brauer, J E Robbers

    Applied and Environmental Microbiology
    |January 1, 1987
    PubMed
    Summary

    Parasexuality was demonstrated in the ergot fungus (Claviceps sp.) using genetically modified strains. This breakthrough enables mitotic analysis for studying ergot genetics and improving desirable traits.

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    Area of Science:

    • Mycology
    • Fungal Genetics
    • Biotechnology

    Background:

    • Ergot fungi (Claviceps sp.) are known for producing clavine alkaloids.
    • Understanding the genetics of Claviceps sp. is crucial for optimizing alkaloid production.
    • Parasexuality, a mode of genetic exchange, has not been clearly demonstrated in this fungus.

    Purpose of the Study:

    • To demonstrate the occurrence of parasexuality in a homokaryotic strain of Claviceps sp. (SD 58).
    • To establish methods for studying Claviceps sp. genetics through mitotic analysis.
    • To explore protoplast fusion for enhancing beneficial characteristics in Claviceps strains.

    Main Methods:

    • Generation of auxotrophic mutants using N-methyl-N'-nitro-N-nitrosoguanidine.
    • Protoplast fusion between doubly auxotrophic strains.
    • Selection of prototrophic fusion products and subsequent induction of segregation.

    Main Results:

    • Stable prototrophic fusion products were isolated.
    • Auxotrophic segregants with novel combinations of traits were recovered.
    • Evidence strongly supports the functioning of a parasexual cycle in Claviceps sp. SD 58.

    Conclusions:

    • Parasexuality can occur in Claviceps sp. strain SD 58.
    • Mitotic analysis is a viable approach for studying ergot genetics.
    • Protoplast fusion offers a pathway for strain improvement in Claviceps.

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