Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
The Tumor Microenvironment02:17

The Tumor Microenvironment

6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A venetoclax-cytarabine-based induction regimen incorporating a translation inhibitor for adult patients with de novo acute myeloid leukemia.

Cancer·2026
Same author

The novel HLA-C*03:652 allele, identified by Sanger dideoxy nucleotide sequencing in a Chinese individual.

HLA·2024
Same author

Treatment failure in a patient infected with <i>Listeria</i> sepsis combined with latent meningitis: A case report.

World journal of clinical cases·2022
Same author

Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis.

Cell metabolism·2021
Same author

Zinc finger and BTB domain-containing protein 46 is essential for survival and proliferation of acute myeloid leukemia cell line but dispensable for normal hematopoiesis.

Chinese medical journal·2020
Same author

Resveratrol: Review on its discovery, anti-leukemia effects and pharmacokinetics.

Chemico-biological interactions·2019

Related Experiment Video

Updated: Jul 4, 2025

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
10:52

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Published on: March 30, 2018

11.2K

SENP3 Promotes Mantle Cell Lymphoma Development through Regulating Wnt10a Expression.

Yan-Ni Ma1,2, Yun-Ding Zou1, Zhi-Long Liu1

  • 1Department of Hematology, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, 400038, China.

Current Medical Science
|January 25, 2024
PubMed
Summary

SUMO-specific protease 3 (SENP3) is upregulated in mantle cell lymphoma (MCL). Inhibiting SENP3 reduces MCL cell growth and promotes apoptosis, suggesting SENP3 as a potential therapeutic target for MCL.

Keywords:
SENP3apoptosiscell proliferationmantle cell lymphoma

More Related Videos

The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

111.8K
Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity
07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

7.5K

Related Experiment Videos

Last Updated: Jul 4, 2025

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
10:52

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Published on: March 30, 2018

11.2K
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

111.8K
Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity
07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

7.5K

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • SUMO-specific protease 3 (SENP3) deconjugates SUMO-2/3, and its dysregulation is implicated in various cancers.
  • The role of SENP3 in mantle cell lymphoma (MCL), an aggressive B-cell lymphoma, is not well understood.

Purpose of the Study:

  • To investigate the role and mechanism of SENP3 in the development of mantle cell lymphoma.
  • To evaluate SENP3 as a potential therapeutic target for MCL.

Main Methods:

  • SENP3 expression analysis in MCL cells and tissues using RT-qPCR, Western blotting, and immunohistochemistry.
  • SENP3 knockdown in MCL cells via short hairpin RNAs, followed by proliferation (CCK-8) and apoptosis (flow cytometry) assays.
  • Mechanism elucidation using mRNA sequencing and in vivo validation in a xenograft nude mouse model.

Main Results:

  • SENP3 expression is significantly upregulated in MCL patient samples and cells.
  • SENP3 knockdown inhibited MCL cell proliferation and induced apoptosis.
  • SENP3 knockdown suppressed the canonical Wnt signaling pathway and Wnt10a expression, and reduced tumor growth in vivo.

Conclusions:

  • SENP3 plays a crucial role in the pathogenesis of MCL.
  • Targeting SENP3 represents a promising therapeutic strategy for mantle cell lymphoma.