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Natural Killer NK and CAR-NK Cell Expansion Method using Membrane Bound-IL-21-Modified B Cell Line

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Reprogramming natural killer cells for cancer therapy.

Kexin Wang¹, Linqin Wang¹, Yiyun Wang¹

¹Bone Marrow Transplantation Center, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province, China; Liangzhu Laboratory, Hangzhou, Zhejiang Province, China; Institute of Hematology, Zhejiang University, Hangzhou, Zhejiang Province, China; Zhejiang Province Engineering Research Center for Stem Cell and Immunity Therapy, Hangzhou, Zhejiang Province, China.

Molecular Therapy : the Journal of the American Society of Gene Therapy

|January 26, 2024

Summary

Natural killer (NK) cell therapy offers an alternative to CAR-T cell therapy for cancer treatment. This review explores strategies to enhance NK cell effectiveness, persistence, and tumor targeting for improved immunotherapy outcomes.

Keywords:

CAR NK cells cancer genetic engineering immunotherapy

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Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Flow Cytometry-based Assay for the Monitoring of NK Cell Functions

Published on: October 30, 2016

Area of Science:

Immunology
Oncology
Biotechnology

Background:

Chimeric antigen receptor (CAR)-T cell therapy shows promise but faces challenges like toxicity and product variability.
Natural Killer (NK) cells are being explored as an alternative immunotherapy due to their unique properties and potential for "off-the-shelf" products.

Purpose of the Study:

To review current clinical trials of antitumor NK cell therapy.
To provide an overview of innovative strategies for reprogramming NK cells to enhance their antitumor functions.
To discuss potential combination therapies for improved cancer treatment.

Main Methods:

Review of current clinical trials involving NK cell therapy.
Exploration of gene-editing technologies and combination approaches to enhance NK cell functions.
Analysis of strategies to improve NK cell persistence, cytotoxicity, trafficking, and ability to overcome the tumor microenvironment.

Main Results:

NK cell therapy, including unedited and reprogrammed cells, has shown early signs of effectiveness against tumors.
Advanced gene-editing and combination strategies are being explored to overcome NK cell limitations.
Improvements in NK cell persistence, cytotoxicity, trafficking, and immune evasion are key research areas.

Conclusions:

NK cells present a promising alternative to CAR-T cell therapy, with potential for "off-the-shelf" applications.
Innovative strategies are crucial for enhancing NK cell antitumor activity and overcoming current limitations.
Further research into gene editing and combination therapies will advance NK cell-based cancer immunotherapy.