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Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
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Valproic acid regulates the miR-155/Jarid2 axis by affecting miR-155 promoter methylation in glioma

Ruixuan Wang1, Yanhong Chen2, Weilu Kuang1

  • 1Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, China.

Acta Biochimica Et Biophysica Sinica
|January 26, 2024
PubMed

Insights

Valproic acid (VPA) impacts glioma treatment by modulating the miR-155/JARID2 axis. VPA upregulates JARID2 and downregulates miR-155, inhibiting glioma cell viability and promoting apoptosis.

Area of Science:

  • Neuro-oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Glioma is the most common adult primary brain tumor with limited curative options.
  • JARID2 enhances glioma sensitivity to temozolomide (TMZ) treatment.
  • MicroRNA-155 (miR-155) is overexpressed in glioma and promotes cell viability while inhibiting apoptosis.

Purpose of the Study:

  • To investigate the role of miR-155 in glioma.
  • To elucidate the regulatory relationship between miR-155 and JARID2.
  • To explore the therapeutic potential of targeting the miR-155/JARID2 axis in glioma.

Main Methods:

  • Bioinformatic prediction and experimental validation of miR-155 targeting JARID2.
  • Analysis of miR-155 and JARID2 expression in clinical glioma specimens and cell lines.
  • In vitro assays assessing cell viability, apoptosis, and in vivo tumor formation.
  • Investigation of Valproic acid (VPA) effects on the miR-155/JARID2 axis and glioma phenotypes.

Main Results:

  • miR-155 targets and inhibits JARID2 expression in glioma cells.
  • miR-155 overexpression enhances glioma cell viability and represses apoptosis.
  • miR-155 inhibition decreases glioma cell viability and enhances apoptosis, an effect partially reversed by JARID2 knockdown.
  • VPA upregulates JARID2, downregulates miR-155 via promoter methylation, inhibits glioma cell viability, and enhances apoptosis.

Conclusions:

  • The miR-155/JARID2 axis plays a critical role in glioma progression.
  • Targeting this axis represents a potential therapeutic strategy for glioma.
  • VPA exerts anti-glioma effects by modulating the miR-155/JARID2 pathway.
Keywords:
JARID2gliomasmethylationmiR-155valproic acid

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