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Frontiers in Bioengineering and Biotechnology
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Summary

This study characterizes lactic acid bacteria and chain-elongating bacteria in waste-to-chemical bioprocesses. Understanding their growth dynamics is key for optimizing caproic acid production from organic waste.

Keywords:
R/K strategiescaproic acidchain-elongating bacteriagranular sludgegrowth parametersproduct inhibition

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Area of Science:

  • Biotechnology and Bioengineering
  • Environmental Science and Engineering
  • Microbiology and Microbial Ecology

Background:

  • Efficient waste management is crucial for a sustainable society.
  • Mixed-culture biotechnology offers a promising route for producing valuable chemicals from organic waste.
  • Knowledge-driven bioprocess development requires detailed insights into microbial community interactions and growth characteristics.

Purpose of the Study:

  • To establish a granular sludge bioprocess for caproic acid production via sugar-based chain elongation.
  • To characterize the growth features of key microbial guilds, specifically lactic acid bacteria and chain-elongating bacteria.
  • To understand the metabolic interactions and growth strategies influencing bioprocess efficiency.

Main Methods:

  • Established a granular sludge bioprocess utilizing sugar-based chain elongation metabolism.
  • Isolated and characterized representative strains of lactic acid bacteria (Limosilactobacillus musocae G03) and chain-elongating bacteria (Caproiciproducens lactatifermentans).
  • Quantified growth rates, biomass yields, half-saturation constants, and determined caproic acid inhibition effects on microbial growth.

Main Results:

  • Lactic acid bacteria exhibited higher growth rates (0.051 h⁻¹) but lower biomass yields (0.120 g/g glucose) compared to chain-elongating bacteria (0.026 h⁻¹, 0.239 g/g glucose), indicating distinct r- and K-strategist behaviors.
  • The half-saturation constant for glucose in L. musocae was 0.35 g/L.
  • A high caproic acid inhibitory concentration of 13.6 g/L was determined for L. musocae, with pre-exposure restoring growth rates at low concentrations.

Conclusions:

  • The differential growth strategies of lactic acid bacteria and chain-elongating bacteria significantly impact sugar-based chain elongation systems.
  • High caproic acid tolerance in lactic acid bacteria is essential for their persistence and function in caproic acid production systems.
  • These findings provide critical insights for optimizing microbial community interactions and designing more efficient bioprocesses for chemical production from waste.