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Updated: Jul 4, 2025
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Association between gut microbiota and peptic ulcer disease, particularly gastric ulcer and duodenal ulcer: a
Zhenhua Dong1, Kai Yu2, Yuchao Xin3
1Department of Gastric and Colorectal Surgery, The First Hospital of Jilin University Changchun, Jilin, China.
Background:
Recent an observational study has suggested a potential connection between gut microbiota (GM) and peptic ulcer diseases (PUDs), particularly gastric ulcer (GU) and duodenal ulcer (DU). However, the causal connection remains unsure.
Methods:
A two-sample Mendelian randomization (MR) is carried out to explore the connection between the GM and DU or GU. Data on the GM comes from the MiBioGend database, and GU or DU data are based on the FinnGen database. One group of single nucleotide polymorphisms (SNPs) (P < 5 × 10-8) are served as instrumental variables (IVs). To obtain a more comprehensive conclusion, the other SNPs (P < 1 × 10-5) are selected as IVs. Inverse variance weighting (IVW) is used to determine the causal relationship.
Results:
At the level of P < 1 × 10-5, the IVW analysis suggests that Clostridiaceae1, Butyriccoccus, and Peptcoccus have harmful effects on GU, while LachnospiraceaeUCG004 and MollicutesRF9 have beneficial effects on GU. Then, in the case of DU, the IVW analysis suggested that Lentisphaeria, Negativicutes, Clostridiaceae1, ClostridiumseMnsustricto1, ErysipelotrichaceaeUCG003, LachnospiraceaeNC2004group, Selenomonadale, Victivallales, and Lentisphaerae have harmful effects, while Catenibacterium, Escherichia.Shigella, LachnospiraceaeUCG008, and Sutterella have beneficial effects. When P < 5 × 10-8, IVW analysis suggests that GM has no significant influence on GU or DU.
Conclusion:
This two-sample MR indicates a causal relationship between GM and GU or DU.
Insights
This study used Mendelian randomization to investigate the link between gut microbiota (GM) and peptic ulcer diseases (PUDs). Certain gut bacteria were found to causally influence gastric ulcers (GU) and duodenal ulcers (DU).
Area of Science:
- Microbiome research
- Gastroenterology
- Genetics
Background:
- Observational studies suggest a link between gut microbiota (GM) and peptic ulcer diseases (PUDs), including gastric ulcers (GU) and duodenal ulcers (DU).
- The causal relationship between GM and PUDs remains unclear.
Purpose of the Study:
- To investigate the potential causal relationship between gut microbiota and the risk of developing gastric ulcers (GU) and duodenal ulcers (DU).
Main Methods:
- A two-sample Mendelian randomization (MR) study was conducted using data from the MiBioGend and FinnGen databases.
- Single nucleotide polymorphisms (SNPs) were used as instrumental variables (IVs) at two significance thresholds (P < 5 × 10-8 and P < 1 × 10-5).
- Inverse variance weighting (IVW) was employed to assess causal effects.
Main Results:
- At P < 1 × 10-5, specific gut bacteria like Clostridiaceae1, Butyriccoccus, and Peptococcus were associated with increased GU risk, while LachnospiraceaeUCG004 and MollicutesRF9 showed protective effects.
- For DU, several bacterial taxa including Lentisphaeria, Negativicutes, and Clostridiaceae1 were linked to increased risk, whereas Catenibacterium, Escherichia.Shigella, LachnospiraceaeUCG008, and Sutterella exhibited protective effects.
- At the stricter threshold (P < 5 × 10-8), no significant causal influence of GM on GU or DU was detected.
Conclusions:
- The two-sample MR analysis provides evidence for a causal relationship between specific gut microbiota compositions and the development of gastric ulcers (GU) and duodenal ulcers (DU).
- Further research is warranted to elucidate the mechanisms underlying these gut microbiota-ulcer associations.
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