Related Experiment Video
Updated: Jul 4, 2025
06:26
Analyzing Ex Vivo Metabolic Flux in Splenic and Cardiac Macrophages and Bone Marrow Monocytes
Published on: March 28, 2025
233
Deciphering metabolic crosstalk in context: lessons from inflammatory diseases
Fenne W M Verheijen1,2, Thi N M Tran1,3, Jung-Chin Chang1
1Division of Cell Biology, Metabolism & Cancer, Department Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, The Netherlands.
Molecular Oncology
|January 26, 2024
Summary
Investigating immune cell metabolism requires considering the microenvironment and spatial organization. This review covers technologies and challenges for studying immunometabolism in disease.
Area of Science:
- Immunology
- Metabolism
- Cell Biology
Background:
- Immune cell metabolism is vital for health and disease, influencing cancer and inflammation.
- The local microenvironment significantly impacts immune cell metabolism.
- Heterogeneity and spatial organization are key factors in immunometabolism.
Purpose of the Study:
- To highlight challenges in investigating metabolic communication.
- To review technologies for studying metabolism in inflamed microenvironments.
- To discuss metabolomics applications and hurdles in clinical practice.
Main Methods:
- Single-cell omics techniques
- Flow cytometry-based methods (Met-Flow, SCENITH)
- Cytometry by time of flight (CyTOF)
- CITE-Seq
- Mass spectrometry imaging
Main Results:
- Current technologies offer insights into immune cell metabolism in complex environments.
- Challenges exist in studying metabolic communication and implementing metabolomics clinically.
- A flowchart is provided to guide immunometabolism research strategies.
Conclusions:
- Understanding immunometabolism is crucial for deciphering disease pathogenesis.
- Technological advancements are improving the study of immune cell metabolism.
- Overcoming clinical implementation hurdles is necessary for broader application of metabolomics.
Keywords:
advanced metabolomics methodsimmunometabolisminflammatory diseasesmetabolic crosstalkmetabolic heterogeneitymicroenvironmentMore Related Videos
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