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Genomic Characterization and Molecular Evolution of Sapovirus in Children under 5 Years of Age

Xiaolei Ji1, Chen Guo2, Yaoyao Dai1

  • 1Key Laboratory of Medicine, Nantong Center for Disease Control and Prevention, 189 Gongnongnan Road, Chongchuan District, Nantong 226007, China.

Viruses
|January 26, 2024
PubMed

Insights

Sapovirus (SaV) causes gastroenteritis in young children. This study identified SaV in 3.93% of samples, sequenced nine genomes, and analyzed their genetic diversity and evolution in China.

Area of Science:

  • Virology
  • Molecular Epidemiology
  • Public Health

Background:

  • Sapovirus (SaV) is a significant cause of acute gastroenteritis, particularly in children under five years old.
  • Understanding the genetic diversity and epidemiology of SaV is crucial for developing effective prevention and control strategies.

Purpose of the Study:

  • To investigate the prevalence and genetic characteristics of Sapovirus in children with acute gastroenteritis in the Nantong region of China.
  • To characterize complete SaV genomes, including the first reported GI.6 strain from mainland China.

Main Methods:

  • Collection and RT-qPCR screening of 712 stool samples from children under five with acute gastroenteritis.
  • Library construction and high-throughput sequencing of SaV-positive samples (Ct < 30).
  • Bioinformatic analysis including BLAST identification, sequence identity analysis, phylogenetic analysis, and population dynamics.

Main Results:

  • Sapovirus was detected in 3.93% (28/712) of the samples.
  • Nine complete SaV genomes were obtained, with eight identified as GI.1 and one as GI.6.
  • Significant nucleotide variations were observed between the identified strains and prototype strains; no recombination events were detected, but potential competitive inhibition between GI.1 lineages was suggested.

Conclusions:

  • This study provides the first complete genome sequence of a GI.6 Sapovirus strain from mainland China, addressing a critical data gap.
  • The findings offer insights into the molecular epidemiology and genetic evolution of Sapovirus in the Nantong region.
  • The data supports enhanced disease prevention, control, and pathogen tracing efforts for Sapovirus in the area.

Keywords:
GI.1GI.6amino acid variationsmolecular evolutionary ratepopulation dynamicssapovirus

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