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An artificial nickel chlorinase based on the biotin-streptavidin technology
Kun Yu1, Kailin Zhang1, Roman P Jakob2
1Department of Chemistry, University of Basel, Mattenstrasse 22, Basel, CH-4058, Switzerland. thomas.ward@unibas.ch.
Abstract:
Herein, we report on an artificial nickel chlorinase (ANCase) resulting from anchoring a biotinylated nickel-based cofactor within streptavidin (Sav). The resulting ANCase was efficient for the chlorination of diverse C(sp3)-H bonds. Guided by the X-ray analysis of the ANCase, the activity of the artificial chlorinase could be significantly improved. This approach opens interesting perspectives for late-stage functionalization of organic intermediates as it complements biocatalytic chlorination strategies.
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