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CircLZIC regulates ox-LDL-induced HUVEC cell proliferation and apoptosis via Micro-330-5p/NOTCH2 axis in

Xingping Men1, Aizhen Hu1, Tingting Xu1

  • 1Department of Cardiology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.

Clinical Hemorheology and Microcirculation
|January 26, 2024
PubMed

Insights

Circular RNA LZIC (circLZIC) promotes atherosclerosis by inhibiting MicroRNA-330-5p, leading to increased NOTCH2 expression and HUVEC cell proliferation, while suppressing apoptosis. This highlights circLZIC as a key regulator in atherosclerosis development.

Area of Science:

  • Cardiovascular Biology
  • Molecular Biology
  • Epigenetics

Background:

  • Atherosclerosis (AS) is a leading cause of cardiovascular disease, driven by chronic inflammation.
  • Circular RNAs (circRNAs) are emerging as critical epigenetic regulators of vascular inflammation and AS progression.
  • Understanding circRNAs that modulate ox-LDL levels is crucial for deciphering AS pathology.

Purpose of the Study:

  • To investigate the role of circLZIC in regulating atherosclerosis (AS) through the MicroRNA-330-5p/NOTCH2 axis.
  • To elucidate the molecular mechanisms by which circLZIC influences human umbilical vein endothelial cell (HUVEC) behavior in the context of AS.

Main Methods:

  • Analysis of circLZIC, MicroRNA-330-5p, and NOTCH2 expression in human AS samples.
  • Cell proliferation (CCK-8) and apoptosis (flow cytometry) assays in HUVECs.
  • RNA interference, dual-luciferase reporter assays, and RIP-qRT-PCR to confirm regulatory interactions.

Main Results:

  • CircLZIC and NOTCH2 were highly expressed in AS samples, while MicroRNA-330-5p was locally expressed.
  • CircLZIC promoted HUVEC proliferation and inhibited apoptosis; its knockdown increased MicroRNA-330-5p, promoted apoptosis, and inhibited NOTCH2.
  • MicroRNA-330-5p targeted NOTCH2, and its overexpression reduced NOTCH2 expression, inhibited apoptosis, and decreased cell activity.

Conclusions:

  • CircLZIC promotes HUVEC proliferation and inhibits apoptosis via the MicroRNA-330-5p/NOTCH2 pathway.
  • CircLZIC plays a significant role in the development of atherosclerosis.
  • The circLZIC/MicroRNA-330-5p/NOTCH2 axis represents a potential therapeutic target for AS.

Keywords:
CircLZICMicro-330-5p/NOTCH2apoptosisatherosclerosisproliferation

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