Human monoclonal antibody recognizing liver-type aldolase B

Area of Science:

• Immunology
• Biochemistry
• Oncology

Background:

• Hybridoma technology enables the production of specific monoclonal antibodies.
• Monoclonal antibodies are crucial tools in biological research and diagnostics.
• Aldolase B is an enzyme with known roles in metabolism and potential links to cancer.

Purpose of the Study:

• To establish and characterize a human hybridoma clone secreting a specific antibody.
• To identify the antigen recognized by the monoclonal antibody 4E3.
• To investigate the potential of this antibody in immunological assays.

Main Methods:

• Human hybridoma (4E3) generation via lymphocyte fusion.
• Immunochemical characterization of the antigen recognized by 4E3.
• Purification of aldolase B and confirmation using ELISA, Western blotting, and SDS-PAGE.
• Assessment of complement activation upon antibody-antigen interaction.

Main Results:

• Hybridoma clone 4E3 consistently secreted IgMk antibody for over a year.
• The antigen recognized by 4E3 exhibited properties consistent with liver-type aldolase B.
• Monoclonal antibody 4E3 demonstrated strong reactivity with purified aldolase B across multiple assays.
• The binding of 4E3 to aldolase B activated the human complement system.

Conclusions:

• The human hybridoma clone 4E3 produces a monoclonal antibody that specifically recognizes human, mouse, and rabbit liver-type aldolase B.
• This antibody is a valuable reagent for studying aldolase B and its potential role in diseases like cancer.
• The antibody's ability to activate complement suggests further utility in immunodiagnostic and immunotherapeutic strategies.