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Pentobarbital discrimination in the mouse.

R L Balster, V C Moser

    Alcohol and Drug Research
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Mice can be trained for drug discrimination research using sodium pentobarbital (PB). This study demonstrates effective training methods and assesses the drug

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    Area of Science:

    • Behavioral pharmacology
    • Neuroscience
    • Drug discrimination research

    Background:

    • Mice are infrequently utilized in drug discrimination studies.
    • Establishing reliable drug discrimination models in rodents is crucial for understanding drug mechanisms.
    • Previous research has primarily focused on pigeons, rats, and monkeys for drug discrimination.

    Purpose of the Study:

    • To develop and validate a drug discrimination procedure in mice using sodium pentobarbital (PB).
    • To assess the pharmacological specificity of the PB stimulus through generalization testing.
    • To determine the feasibility of using typical drug discrimination paradigms with mice.

    Main Methods:

    • Mice were trained using a two-lever operant procedure under a fixed-ratio 20 schedule for milk reinforcement.

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  • Initial training involved 10 mg/kg PB or saline, later adjusted to 15 mg/kg PB for improved stimulus control.
  • Pharmacological specificity was evaluated through generalization tests with methohexital, ethanol, chlorpromazine, and morphine.

    • Main Results:

    • A mean accuracy of over 90% was achieved after adjusting the PB dose to 15 mg/kg.
    • The PB stimulus exhibited a time-dependent effect, with peak discrimination occurring at 5-20 min post-injection.
    • Mice generalized the PB stimulus to methohexital and ethanol, but not to morphine or chlorpromazine.

    Conclusions:

    • A robust drug discrimination procedure for sodium pentobarbital in mice was successfully established.
    • The findings confirm the utility of mice in drug discrimination research, mirroring results from other species.
    • Standard drug discrimination protocols are adaptable for use with mice, expanding research capabilities.