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Updated: Jul 4, 2025
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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
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Evolution of LC-MS/MS in clinical laboratories
Songlin Yu1, Yutong Zou1, Xiaoli Ma1
1Department of Laboratory Medicine, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Science, Beijing, China.
Clinica Chimica Acta; International Journal of Clinical Chemistry
|January 27, 2024
Summary
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) offers clinical advantages but faces challenges in biomarker selection, automation, and standardization. Addressing these issues through utility-driven approaches and collaborative efforts will enhance its clinical integration.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Biomarker Discovery
Background:
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly recognized for its clinical utility.
- Widespread adoption is currently limited by challenges in analyte selection, automation, and standardization.
Purpose of the Study:
- To review the current challenges hindering the clinical application of LC-MS/MS.
- To propose potential resolutions for overcoming these limitations and facilitating broader adoption.
Main Methods:
- Comprehensive literature review of LC-MS/MS applications and challenges in clinical practice.
- Analysis of current limitations including analyte selection, automation, reference intervals, and quality control.
- Discussion of proposed solutions focusing on utility-driven biomarker selection, methodological innovation, standardization, and quality assurance.
Main Results:
- Key challenges identified include inappropriate biomarker selection, insufficient automation, lack of standardized reference intervals, and inadequate quality control programs.
- Proposed resolutions emphasize prioritizing practical utility in biomarker selection, developing high-throughput automation, establishing multi-center reference intervals, and creating commercial quality control materials.
- Harmonization and standardization efforts are crucial for clinical LC-MS/MS, with commercial kits and LDTs expected to coexist.
Conclusions:
- Addressing challenges in analyte selection, automation, and standardization is vital for the clinical integration of LC-MS/MS.
- Future advancements will focus on simplification, automation, intelligence, and standardization, enhancing its routine use for clinicians and technicians.
- Collaborative efforts among professionals are essential for the successful clinical implementation of LC-MS/MS.
Keywords:
Analyte selectionAutomationClinical laboratoryLiquid chromatography–tandem mass spectrometryQuality controlReference intervalMore Related Videos
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