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Related Experiment Video

Updated: Jul 4, 2025

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

Zebrafish prss59.1 is involved in chorion development.

Md Rezanujjaman¹, Theeranukul Pachoensuk¹, Md Forhad Hossain¹

¹Department of Bioscience, Graduate School of Science and Technology, National University Corporation, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.

General and Comparative Endocrinology

|January 28, 2024

Summary

The prss59.1 gene is crucial for zebrafish egg development, enabling proper chorion expansion and structure. Its absence leads to smaller chorions and reduced embryo survival rates.

Keywords:

Chorion Fertilization Prss59.1 Zebrafish

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Last Updated: Jul 4, 2025

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

Dissection of Larval Zebrafish Gonadal Tissue

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Published on: April 26, 2017

Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants

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Published on: December 21, 2021

Area of Science:

Developmental Biology
Genetics
Reproductive Biology

Background:

The prss59.1 gene was identified as upregulated during zebrafish ovulation.
Prss59.1 is a trypsin-like proteolytic enzyme.

Purpose of the Study:

To investigate the function of the prss59.1 gene in zebrafish.
To determine the role of Prss59.1 in chorion formation and egg activation.

Main Methods:

CRISPR/Cas9 gene editing to create prss59.1 knockout zebrafish.
Phenotypic analysis of mutant zebrafish.
Transmission electron microscopy (TEM) for chorion structure evaluation.

Main Results:

prss59.1 knockout zebrafish exhibited smaller chorions compared to wild type.
Homozygous mutants showed significantly reduced survival rates.
Abnormalities in chorion structure, including pore size and knob-like structures, were observed in mutants.
Prss59.1 was localized to the chorionic knob-like structures.

Conclusions:

Prss59.1 is essential for chorion expansion and proper chorion formation in zebrafish.
The gene plays a critical role in egg activation and subsequent embryo development.