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Updated: Jul 26, 2026
07:05
Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer
Published on: September 22, 2015
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Printable oxygen-generating biodegradable scaffold for thicker tissue-engineered medical products
Zihan Pei1, Kevin Montagne2, Ayaka Namiki1
1Department of Bioengineering, The University of Tokyo, Tokyo, Japan.
Artificial Organs
|January 29, 2024
Summary
New 3D-printed scaffolds release oxygen, reducing cell death in tissue engineering. These biodegradable scaffolds show promise for regenerative medicine applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Increasing demand for thick, vascularized tissue-engineered constructs necessitates novel strategies.
- Limitations in gas diffusion and transport hinder transplanted tissue and device viability.
- Oxygen-releasing biomaterials offer a potential solution for improved tissue survival.
Purpose of the Study:
- To develop a novel 3D-printable scaffold for tissue engineering.
- To incorporate oxygen-generating capabilities into biodegradable scaffolds.
- To assess the efficacy of oxygen-releasing scaffolds in reducing cell death.
Main Methods:
- Fabrication of biodegradable polycaprolactone (PCL) scaffolds containing calcium peroxide (CPO).
- Utilized 3D printing technology for scaffold construction with arbitrary shape formation.
- Cultured osteoblast progenitor cells (MC3T3-E1) under hypoxic conditions on the fabricated scaffolds.
Main Results:
- Scaffolds successfully incorporated calcium peroxide (CPO) for oxygen generation.
- 3D printing enabled the creation of complex scaffold architectures.
- Reduced cell death was observed in MC3T3-E1 cells cultured on oxygen-releasing scaffolds under hypoxia.
Conclusions:
- Biodegradable, oxygen-releasing scaffolds fabricated via 3D printing show potential for tissue engineering.
- The developed scaffolds can mitigate hypoxic conditions, improving cell survival.
- This technology may advance regenerative medicine by enhancing construct viability.
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