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Updated: Aug 12, 2026

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
Published on: July 31, 2010
Free energy couplings between ligand binding and subunit association in hemoglobin are of first order
Abstract:
The calculations presented in a recent paper [Johnson, M. L. (1986) Biochemistry 25, 791-797] to the effect that the free energy couplings between oxygen binding and subunit association in hemoglobin A can be of either first or second order are examined. The fitting of the experimental data to a system with second-order couplings carried out by Johnson belongs to a tetramer in which, in contradistinction to hemoglobin A, oxygen binding promotes subunit association.
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