Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Base Pairing02:27

DNA Base Pairing

27.4K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
27.4K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K
Sanger Sequencing01:57

Sanger Sequencing

754.4K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
754.4K
Labeling DNA Probes03:31

Labeling DNA Probes

8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

14.1K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Carbon Cage Nanosensors for Selective Detection of Toxic Gas Molecules.

ACS omega·2026
Same author

Robust nanotube-based nanosensor designed for the detection of explosive molecules.

Nanoscale advances·2024
Same author

Towards nanotube-based sensors for discrimination of drug molecules.

Physical chemistry chemical physics : PCCP·2023
Same author

Defect-Induced Transport Enhancement in Carbon-Boron Nitride-Carbon Heteronanotube Junctions.

The journal of physical chemistry letters·2023
Same author

Thermoelectric Response Enhanced by Surface/Edge States in Physical Nanogaps.

Materials (Basel, Switzerland)·2023
Same author

Discriminating sensing of explosive molecules using graphene-boron nitride-graphene heteronanosheets.

RSC advances·2022

Related Experiment Video

Updated: Jul 4, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

983

Selective Sensing of DNA Nucleobases with Angular Discrimination.

Laith A Algharagholy1, Víctor Manuel García-Suárez2, Sawsan S Abaas3

  • 1Department of Physics, College of Science, University of Sumer, Al-Rifai, 64005 Thi-Qar, Iraq.

ACS Omega
|January 29, 2024
PubMed
Summary

This study introduces a novel carbon nanotube sensor capable of distinguishing individual DNA nucleobases (A, C, G, T) and their orientation. This advancement promises more precise DNA sequencing for improved genomics and personalized medicine.

More Related Videos

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

9.5K
A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

8.1K

Related Experiment Videos

Last Updated: Jul 4, 2025

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

983
Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

9.5K
A Polyaniline-based Sensor of Nucleic Acids
07:58

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

8.1K

Area of Science:

  • Nanotechnology
  • Genomics
  • Computational Science

Background:

  • Accurate DNA nucleobase sensing is crucial for genomics and personalized medicine.
  • Existing methods face challenges in achieving high selectivity and precision.
  • DNA sequencing advancements rely on differentiating individual nucleobases and their spatial arrangement.

Purpose of the Study:

  • To develop a nanoscale sensor for fast and precise selective sensing of DNA nucleobases.
  • To demonstrate the sensor's ability to discriminate between the four types of nucleobases (A, C, G, T).
  • To show the sensor's capability in identifying the angle of orientation of each nucleobase.

Main Methods:

  • Utilized first-principle and quantum transport simulations.
  • Calculated transmission, conductance, and current of a carbon nanotube-based sensor.
  • Simulated sensor performance with four nucleotides (A, C, G, T) at various orientations (0°, 90°, 180°, 270°).

Main Results:

  • The carbon nanotube sensor effectively discriminated between the four DNA nucleobases.
  • The system successfully differentiated the angle of orientation for each nucleobase.
  • Observed nontrivial changes in transmission due to the interaction between the nucleotide's phosphate group and the nanotube wall.

Conclusions:

  • The developed nanoscale sensor offers a method for highly precise DNA chain sequencing.
  • The sensor's performance is attributed to specific electronic structure distortions caused by nucleotide-phosphate interactions.
  • This technology has the potential to significantly advance genomics and medical diagnostics.