Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Diet Quality and Dementia Risk in Older Adults With Alzheimer Pathology.

JAMA network open·2026

Same author

A drug repurposing screen identifies antiviral compounds against Puumala Orthohantavirus.

Scientific reports·2026

Same author

Blood biomarkers of Alzheimer's disease and 15-year decline in cognitive and motor functions in older adults.

Journal of internal medicine·2026

Same author

Anemia and Blood Biomarkers of Alzheimer Disease in Dementia Development.

JAMA network open·2026

Same author

Signs of Cross-Reactivity Between SARS-CoV-2 and Other Coronaviruses in a Pre-Vaccine Swedish School Setting.

Acta paediatrica (Oslo, Norway : 1992)·2026

Same author

Optimizing T cell responses of targeted peptide antigen delivery by modulating antigen processing through amino acid exchange.

International journal of biological macromolecules·2026

Search research articles

Related Experiment Video

Updated: Jul 4, 2025

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy.

Maryam Sahi¹, Sarah Andersson¹, Cecilia Mattson¹

¹Affinity Proteomics-Stockholm Unit, SciLifeLab, Division of Affinity Proteomics, Department of Protein Science, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), KTH Royal Institute of Technology.

Journal of Visualized Experiments : Jove

|January 29, 2024

Summary

This study presents a novel flow cytometry assay for detecting intact severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles. The method uses multiplex technology to simultaneously analyze multiple surface protein epitopes, aiding antiviral and vaccine development.

More Related Videos

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Published on: February 10, 2022

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

Related Experiment Videos

Last Updated: Jul 4, 2025

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Published on: February 10, 2022

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19

Published on: February 16, 2022

Area of Science:

Virology
Immunology
Biotechnology

Background:

Enveloped viruses utilize surface membrane proteins for critical functions like cell attachment, fusion, and pathogenesis.
Viral surface proteins are key targets for developing antivirals and vaccines.
Accurate detection of intact viral particles is crucial for understanding viral behavior and developing countermeasures.

Purpose of the Study:

To describe a novel dual-reporter flow cytometric protocol for investigating surface proteins on intact SARS-CoV-2 particles.
To demonstrate the capability of multiplex technology for triple detection of viral particles using independent affinity reactions.
To validate the assay's performance for detecting viral surface protein epitopes and its potential applications.

Main Methods:

Utilized magnetic beads conjugated with recombinant human angiotensin-converting enzyme-2 (ACE2) to capture SARS-CoV-2 viral particles.
Employed a dual-reporter flow cytometry system with R-phycoerythrin (PE) and Brilliant Violet 421 (BV421) labeled detection reagents.
Applied antibody fragments targeting different Spike (S1) protein epitopes for proof-of-concept validation.

Main Results:

Achieved triple detection of viral particles with high specificity, confirming the capture of intact SARS-CoV-2.
Generated dose-dependency curves with good assay performance (replicate coefficient variances <14%).
Demonstrated parallel detection of two distinct viral surface protein epitopes.

Conclusions:

The developed flow cytometry protocol enables high-multiplex, high-throughput profiling of enveloped virus surface proteins.
The assay can reliably detect active, intact viral particles and assess antibody/antiviral drug specificity.
This method holds potential for applications beyond SARS-CoV-2, including other extracellular vesicles and bioparticles.