Related Experiment Video
Updated: Jul 4, 2025

Individualized Reconstitution of Human Milk Microbiota: A Feasible Approach in Real-World Settings
Published on: February 7, 2025
Temperature curve of raw human milk heated by different methods: experimental study
Ana Cristina Freitas de Vilhena Abrão1, Gisele de Jesus Schmidt2, Maria José Guardia Mattar3
1Universidade Federal de São Paulo, Escola Paulista de Enfermagem, Departamento Enfermagem na Saúde da Mulher, São Paulo, SP, Brazil.
Heating human milk using a water bath or microwave quickly reaches body temperature, crucial for neonatal intensive care units. Room temperature is insufficient for adequate warming of human milk.
Area of Science:
- Biomedical Engineering
- Nutritional Science
- Pediatrics
Background:
- Accurate warming of human milk is critical for neonatal nutrition and safety.
- Different heating methods and volumes may affect the final temperature and nutritional integrity of human milk.
- Understanding thermal behavior is essential for clinical practice in neonatal intensive care units.
Purpose of the Study:
- To analyze the temperature curves of raw or pasteurized human milk subjected to various heating methods.
- To determine the optimal heating conditions for human milk in a clinical setting.
Main Methods:
- Human milk samples (5-100 ml) were heated using water bath and microwave methods between 2016 and 2021.
- Temperature changes were recorded over time for different volumes and heating settings.
- Descriptive statistics were employed to analyze the thermal data.
Main Results:
- Water bath and microwave heating brought human milk close to body temperature.
- Human milk heated in a water bath at 40°C reached body temperature within 3-5 minutes for smaller volumes.
- Microwave heating at 50% power effectively heated nearly all tested volumes to the target temperature.
- Room temperature (21°C) did not achieve adequate warming.
Conclusions:
- Temperature curves for human milk were successfully constructed for different heating methods.
- The study provides data on human milk heating behavior, considering volume, method, time, and temperature.
- Findings are applicable to the administration of human milk in neonatal intensive care units.
Related Concept Videos
Assessing Body Temperature - Rectal
Follow these steps for rectal temperature assessment:
Step 1: Perform hand hygiene and don clean gloves to prevent cross-infection.
Step 2: Position the patient in a side-lying position to better visualize the rectal...
Temperature Measurement Sites
Oral: When assessing oral temperature, the thermometer tip should be placed under the tongue in the posterior sublingual pocket. It offers accurate readings and can be...
Assessing Body Temperature - Oral
Step 1:
Start by practicing proper hand hygiene to prevent the spread of microorganisms.
Step 2:
Take the thermometer out of the charging unit, switch it on, and wait for the ready sign.
Step 3:
Gently slide the probe cover until a click is heard. This simple action prevents cross-contamination and ensures the correct placement of the probe cover.
Step 4:
Instruct the patient to open their mouth and place...
Equipments Used to Measure Body Temperature
Glass-bulb Thermometer:
Glass-bulb thermometers are hollow glass tubes with a bulb tip containing liquid such as ethanol or mercury. Historically, glass bulb mercury thermometers were the standard device to measure body temperature. Today, mercury thermometers are prohibited in many countries due to the hazardous effects of mercury and the risk of exposure if the glass bulb breaks. In general,...
Assessing Body Temperature - Tympanic membrane
Step 1: Begin by practicing good hand hygiene to prevent the transmission of microorganisms.
Step 2: Turn on the thermometer and wait until the ready sign appears on the screen to ensure accurate measurement.
Step 3: Slide the probe cover in place to prevent cross-contamination.
Step 4: Instruct the patient to tilt their head to the side for comfort and check for cerumen...
Factors Affecting Body Temperature
Factors may include:

