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Updated: Jul 4, 2025

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

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Trifunctional Sphinganine: A New Tool to Dissect Sphingolipid Function.

Scotland Farley^1,2, Frank Stein³, Per Haberkant³

¹Department of Chemical Physiology & Biochemistry, Oregon Health & Science University, Portland, Oregon 97239, United States.

ACS Chemical Biology

|January 29, 2024

Summary

This summary is machine-generated.

Researchers explored the distinct cellular roles of sphingosine and sphinganine, two vital lipids. Novel probes revealed significant differences in their protein interactions and cellular distributions, clarifying their unique functions.

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Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Area of Science:

Lipid biology
Cellular signaling
Biochemistry

Background:

The precise functions and cellular biology of sphingolipids, specifically sphingosine and sphinganine, remain largely unclear.
While sphingosine's signaling pathways are partially understood, sphinganine is primarily characterized by its lack of similar signaling activities.

Purpose of the Study:

To elucidate the distinct spatiotemporal distributions and protein interactomes of sphingosine and sphinganine within cells.
To establish a foundation for understanding the mechanistic differences and cellular roles of these critical lipids.

Main Methods:

Preparation of multifunctionalized lipid derivatives differing by a single double bond.
In-cell photo-crosslinking to create lipid-protein conjugates.
Click chemistry for extraction and subsequent proteomic analysis of interacting proteins.

Main Results:

Novel lipid probes successfully defined the spatiotemporal localization of sphingosine and sphinganine.
Systematic mapping of protein interactomes revealed significant differences in proteins associated with each lipid.
Distinct cellular processes mediated by sphingosine and sphinganine were highlighted through identified protein partners.

Conclusions:

Significant mechanistic differences exist between sphingosine and sphinganine at the cellular level.
This study provides essential tools and foundational data for future investigations into sphingolipid cellular functions.