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Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

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Temporary Immersion Bioreactors for Sugarcane Multiplication and Rooting.

José Luis Spinoso-Castillo1, María Karen Serrano-Fuentes1, Monserrat Sorcia-Morales2

  • 1Postgraduate College-Campus Cordoba, Amatlan de los Reyes, Veracruz, Mexico.

Methods in Molecular Biology (Clifton, N.J.)
|January 29, 2024
PubMed
Summary

Temporary immersion systems enhance sugarcane micropropagation, improving multiplication and rooting rates. This method reduces labor and culture time for efficient, high-quality plant production.

Keywords:
AcclimatizationMicropropagationPlant tissue cultureShoot proliferation

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Area of Science:

  • Agricultural Science
  • Plant Biotechnology

Background:

  • Sugarcane is a vital global crop for sugar, ethanol, and by-products.
  • Traditional micropropagation methods can be labor-intensive and costly.

Purpose of the Study:

  • To evaluate the efficacy of temporary immersion systems for sugarcane micropropagation.
  • To optimize sugarcane plant development and quality using bioreactors.

Main Methods:

  • Utilized temporary immersion systems (semi-automated bioreactors) for sugarcane tissue culture.
  • Implemented a protocol involving specific immersion times and frequencies in a liquid culture medium.

Main Results:

  • Achieved successful multiplication and rooting of sugarcane plantlets.
  • Demonstrated improved multiplication rates and enhanced rooting compared to conventional methods.
  • Observed reduced culture time, labor requirements, and reagent usage.

Conclusions:

  • Temporary immersion bioreactors offer an efficient alternative for large-scale sugarcane propagation.
  • This technology leads to faster development, better quality plants, and high acclimatization survival rates.