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A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

Published on: October 21, 2010

Proposal for a screening protocol for Candida auris colonization.

S E Leonhard¹, G M Chong¹, D E Foudraine¹

¹Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, Rotterdam, The Netherlands.

The Journal of Hospital Infection

|January 29, 2024

Summary

This summary is machine-generated.

A new polymerase chain reaction (PCR) screening protocol effectively identifies Candida auris colonization in hospitalized patients. This method aids in controlling the spread of this multidrug-resistant yeast in healthcare settings.

Keywords:

Candida Fungal drug resistance Hospitals Molecular diagnostic techniques

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Area of Science:

Medical Mycology
Infectious Diseases
Clinical Microbiology

Background:

Candida auris is a multidrug-resistant yeast causing severe hospital infections.
Its global spread is concerning, with biofilms enabling persistent colonization and environmental contamination.
Difficulties in identifying C. auris via standard cultures pose challenges for infection control.

Purpose of the Study:

To develop and validate a rapid screening protocol for C. auris colonization.
To combine in-house polymerase chain reaction (PCR) with optimized culture methods for accurate detection.

Main Methods:

Developed C. auris-specific primers and a probe targeting the ITS region.
Validated PCR assay with 12 C. auris isolates and 103 other Candida species isolates, achieving 100% accuracy.
Determined the assay's limit of detection to be approximately four cells per PCR.

Main Results:

Screening protocol implemented in February 2023 for high-risk patients.
In eight months, 199 patients were screened, identifying seven positive cases (4% prevalence).
The protocol involved collecting swabs from multiple body sites.

Conclusions:

The developed PCR-based screening protocol shows promise for controlling C. auris in hospital environments.
Early identification of C. auris colonization can aid in implementing timely infection control measures.