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Updated: Jul 4, 2025
10:18
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
Published on: May 28, 2019
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The CRISPR/Cas9 system forms a condensate in the yeast nucleus
Sara Medina-Suárez1,2, Félix Machín1,2,3
1Unidad de Investigación, Hospital Universitario Nuestra Señora de Candelaria, Santa Cruz de Tenerife, Canary Islands, Spain.
Micropublication Biology
|January 30, 2024
Summary
CRISPR/Cas9 gene editing
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- CRISPR/Cas9 technology is a powerful tool for genetic engineering.
- The nuclear behavior of Cas9 in eukaryotic cells, especially yeast, is not well understood.
Purpose of the Study:
- To investigate the nuclear dynamics of Cas9 in Saccharomyces cerevisiae.
- To understand the localization and distribution of Cas9 within the yeast nucleus.
Main Methods:
- Constructed yeast strains expressing fluorescently tagged Cas9 variants.
- Observed Cas9 localization and distribution using microscopy over time.
Main Results:
- Fluorescently tagged Cas9 accumulated in the nucleus of yeast cells.
- Cas9 exhibited non-uniform nucleoplasmic distribution, forming a condensate.
- This Cas9 condensate frequently co-localized with the nucleolus and associated with the target DNA site.
Conclusions:
- The study elucidates the nuclear dynamics of Cas9 in yeast.
- Findings suggest Cas9 forms a dynamic condensate in the nucleus, interacting with the nucleolus and target DNA.
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