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SIMULATION MODELING OF A HIGH-THROUGHPUT OYSTER CRYOPRESERVATION PATHWAY
Sarah Bodenstein1, Isabelina Nahmens2, Terrence R Tiersch1
1Aquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, LA 70820.
Journal of Shellfish Research
|January 30, 2024
Summary
Oyster genetic resources face threats from mortality. This study models cryopreservation protocols to improve logistical complexities and aid in developing vital oyster germplasm repositories for aquaculture.
Area of Science:
- Aquaculture
- Conservation Biology
- Bioengineering
Background:
- Oyster genetic resources in Louisiana and the Gulf of Mexico are threatened by high unexplained mortality.
- Germplasm repositories are crucial for protecting genetic resources and supporting breeding programs in agriculture.
- Developing oyster repositories is essential but hindered by logistical challenges despite advances in cryopreservation.
Purpose of the Study:
- To bridge the gap between cryopreservation research and practical repository development in oyster aquaculture.
- To model and analyze a high-throughput cryopreservation protocol for oysters.
- To identify and address logistical complexities in establishing oyster germplasm repositories.
Main Methods:
- Defined and mapped steps of a high-throughput cryopreservation protocol using a process flow diagram.
- Developed a simulation model incorporating time study data to analyze the cryopreservation process.
- Created model variations with alternative devices to identify and mitigate process bottlenecks.
Main Results:
- The simulation model accurately represented the oyster cryopreservation process.
- Key performance parameters identified include the number of straws frozen per oyster, batch size, and operator count.
- Strategic device selection significantly improved process efficiency and throughput.
Conclusions:
- Simulation modeling offers a valuable tool for optimizing cryopreservation pathways and repository systems for aquatic species.
- The study provides insights into structuring and operating effective cryopreservation processes for oyster aquaculture.
- Further research is needed to analyze the impact of production scale on cryopreservation efficiency.
