Carbachol changes spike-generation properties of GH3 pituitary cells

Membrane effects of carbachol (50 microM) on GH3 pituitary cells were studied using whole-cell type electrophysiological techniques. Carbachol (50 microM) decreased the threshold for generation of spontaneous and current-induced action potentials and prolonged their duration. It is suggested that carbachol blocks potassium channels in GH3 pituitary cells, and that the effect is mediated via activation of muscarinic cholinergic receptors.