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Updated: Jul 4, 2025
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Sperm Collection of Differential Quality Using Density Gradient Centrifugation
Published on: November 29, 2018
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Swim-up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm
Kenji Amano1, Satoko Oigawa1, Koichiro Ichizawa1
1Reproduction Center, Toho University Omori Medical Center Tokyo Japan.
Reproductive Medicine and Biology
|January 30, 2024
Summary
The swim-up method shows lower sperm DNA fragmentation and higher motility compared to density gradient centrifugation. However, density gradient centrifugation yields a better recovery rate of progressive motile sperm.
Area of Science:
- Reproductive biology
- Andrology
- Sperm processing techniques
Background:
- Sperm DNA fragmentation (SDF) impacts male fertility.
- Sperm processing methods like swim-up and density gradient centrifugation (DGC) are used to select motile sperm for assisted reproductive technologies (ART).
- Evaluating the effects of these methods on SDF and sperm quality is crucial for optimizing ART outcomes.
Purpose of the Study:
- To compare the efficacy of the swim-up method versus density gradient centrifugation (DGC) in reducing sperm DNA fragmentation (SDF).
- To assess the impact of these sperm processing techniques on sperm progressive motility and the recovery rate of progressive motile sperm.
Main Methods:
- Nineteen normozoospermic samples with high motile sperm counts were analyzed.
- Sperm DNA fragmentation (SDF), progressive motility, and progressive motile sperm count were measured before and after processing.
- Samples were processed using either the swim-up method or density gradient centrifugation (DGC).
Main Results:
- Sperm DNA fragmentation (SDF) was not significantly different in unprocessed, swim-up, or density gradient centrifugation (DGC) samples initially.
- However, SDF was significantly lower in swim-up processed samples compared to DGC processed samples.
- Sperm progressive motility was significantly higher in both swim-up and DGC processed samples compared to unprocessed samples, with swim-up showing superior motility.
- The recovery rate of progressive motile sperm was significantly lower with the swim-up method compared to DGC.
Conclusions:
- The swim-up method is superior for reducing sperm DNA fragmentation (SDF) and enhancing sperm progressive motility.
- Density gradient centrifugation (DGC) offers a better recovery rate of progressive motile sperm.
- The choice between swim-up and DGC may depend on the specific goals of sperm processing in fertility treatments.
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