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Enhancing Bioethanol Production from Rice Straw through Environmentally Friendly Delignification Using Versatile
Kenneth Sze Kai Teo1,2, Keiko Kondo1,3,4, Sadat Mohamed Rezk Khattab1,5
1Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Journal of Agricultural and Food Chemistry
|January 30, 2024
Summary
Versatile peroxidase (VP) effectively removed 35% of lignin from rice straw (RS). This eco-friendly pretreatment significantly boosted glucose and bioethanol yields by up to 1.5-fold and 1.4-fold, respectively.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Renewable Energy
Background:
- Rice straw (RS) is a carbohydrate-rich agricultural residue with high bioethanol potential.
- Lignin in RS hinders carbohydrate accessibility, limiting efficient bioethanol conversion.
- Enzymatic delignification offers a promising, eco-friendly pretreatment strategy.
Purpose of the Study:
- To express and utilize versatile peroxidase (VP) for rice straw delignification.
- To investigate the structural changes in rice straw post-VP treatment.
- To evaluate the impact of VP-mediated delignification on saccharification and bioethanol yields.
Main Methods:
- Expression of versatile peroxidase (VP) in *Pichia pastoris*.
- Delignification of rice straw using VP in a membrane bioreactor at 30 °C.
- Analysis of delignified rice straw using Klason lignin assay, SEC, FTIR, and SEM.
- Comparison of saccharification and fermentation yields between VP-treated (VP-RS) and untreated (Ctrl-RS) rice straw.
Main Results:
- VP treatment achieved 35% delignification of rice straw.
- Structural analysis confirmed significant changes in RS due to VP-mediated delignification.
- VP-RS showed enhanced glucose production (up to 1.5-fold) and bioethanol yields (up to 1.4-fold) compared to Ctrl-RS.
Conclusions:
- Versatile peroxidase is effective for delignifying rice straw.
- VP-mediated delignification significantly improves bioethanol production efficiency.
- This enzymatic approach presents a sustainable method for valorizing agricultural residues.
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