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Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

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Quantifying Gut Microbial Short-Chain Fatty Acids and Their Isotopomers in Mechanistic Studies Using a Rapid, Readily

Cheng-Yu Charlie Weng¹, Christopher Suarez¹, Shawn Ehlers Cheang¹

¹Department of Chemistry, University of California Davis, Davis, California 95616, United States.

Analytical Chemistry

|January 30, 2024

Summary

This study introduces a new UHPLC-QqQ-MS method for accurately quantifying short-chain fatty acids (SCFAs) produced by gut microbes. This advancement aids research into gut health, microbiome interactions, and precision nutrition.

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Area of Science:

Microbiology
Analytical Chemistry
Gastroenterology

Background:

Short-chain fatty acids (SCFAs) are key gut microbial fermentation products influencing host physiology and health.
Existing methods for SCFA detection and quantitation are insufficient for complex gut-host-health studies.
SCFAs are implicated in conditions like irritable bowel syndrome (IBS).

Purpose of the Study:

To develop a sensitive, rapid, and expandable UHPLC-QqQ-MS platform for SCFA quantitation.
To enable precise analysis of gut-microbially derived SCFAs and related metabolites.
To support research in gut-microbiome and precision nutrition.

Main Methods:

Development of a UHPLC-QqQ-MS platform utilizing 2-PA derivatization.
Quantitation of SCFAs, related metabolites, and isotopically labeled standards.
Application of the platform to mouse cecal contents, human fecal bioreactors, and fermentation studies.

Main Results:

A sensitive and rapid UHPLC-QqQ-MS method for SCFA quantitation was successfully established.
The platform demonstrated utility in analyzing SCFAs from various biological samples.
Isotopically labeled dietary carbohydrates were used to trace SCFA production.

Conclusions:

The developed UHPLC-QqQ-MS platform is an invaluable tool for gut-microbiome research.
This method enhances the ability to study the gut-host-health paradigm.
The workflow supports the expanding fields of microbiome research and precision nutrition.