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A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
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Tutorial on compressed ultrafast photography
Yingming Lai1, Miguel Marquez1, Jinyang Liang1
1Université du Québec, Institut National de la Recherche Scientifique, Centre Énergie Matériaux Télécommunications, Laboratory of Applied Computational Imaging, Varennes, Québec, Canada.
Journal of Biomedical Optics
|January 31, 2024
Summary
Compressed ultrafast photography (CUP) offers the fastest single-shot imaging, capturing transient events at trillions of frames per second. This review details CUP
Area of Science:
- Optics and Photonics
- Biomedical Imaging
- Computational Imaging
Background:
- Compressed Ultrafast Photography (CUP) represents a significant advancement in single-shot imaging technology.
- CUP integrates compressed sensing with streak imaging to achieve extremely high frame rates.
Approach:
- This tutorial provides a comprehensive review of CUP, covering fundamental methods, system implementations, and biomedical applications.
- It includes step-by-step guidelines for CUP's forward model and image reconstruction algorithms, with sample codes in Matlab and Python.
- Hardware implementation details, including key components and their limitations, are also discussed.
Key Points:
- CUP achieves imaging speeds from thousands to trillions of frames per second with micrometer-level spatial resolution.
- The technique enables broad spectral range sensing for capturing transient events in a single exposure.
- Sample codes and illustrations are provided for understanding CUP's forward model and reconstruction algorithms.
Conclusions:
- CUP is a state-of-the-art ultrafast imaging technology with advanced capabilities and versatility.
- Its application in biomedicine facilitates unprecedented observations and novel research avenues.
- Future prospects include technical enhancements and broader adoption in investigating complex biomedical processes.
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