Hierarchical plant extracts in silver nanoparticles preparation: Minuscular survey to achieve enhanced bioactivities

Extracts obtained from M. longifolia (Lamiaceae) and R. ellipticus (Rosaceae) were selected to utilize in the reduction and stabilization of silver nanoparticles (AgNPs) for achieving remarkable bioactivities. In brief, the cytotoxic potential of the as synthesize AgNPs was high at higher concentrations. In DPPH assay, maximum antioxidant potential was shown by AgNPs synthesized from M. longifolia. Meanwhile, Methanolic extracts exhibited more antioxidant potential than chloroform based extracts. Further, brine shrimp lethality assay was carried out to achieve 34.6 μg/mL & 25.65 μg/mL LD₅₀ values against the NPs prepared from M. and R., respectively. In addition, antioxidant activities were carried by ABTS Radical cation assay where 38.6 μg/mL and 47 μg/mL IC₅₀ values were obtained for the NPs obtained from M. longifolia and R. ellipticus, respectively. Reducing power assay (0.370-0.15 and 0.37-0.26 mean absorbance) and DPPH (% scavenging: 88.91-46.48 and 88.91-44.78) percentages were recorded for M. and R. synthesized AgNPs, respectively. In brief, M. longifolia functionalized particles performed better in comparison to R. ellipticus treated particles. In addition, the nano assembly dispersed in polar solvent demonstrated better results in comparison to non-polar solvents. In conclusion, the as synthesized AgNPs were better in bioactivities than crude extracts of the selected plants. In future, this work could be extended to isolating active components for the nanofabrication of biologically intelligent nanoparticles for pharmacological interest. In the proposed investigation, the purified bioactivities fractions would be highlighted for further consideration in various medical treatments.