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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

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A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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Related Experiment Video

Updated: Jul 4, 2025

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
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Fluorescence visualization for cancer DETECTION: EXPERIENCE and perspectives.

Yaroslav Kravchenko1,2, Kateryna Sikora1, Andrew Awuah Wireko1

  • 1Sumy State University, Sumy, Ukraine.

Heliyon
|January 31, 2024
PubMed
Summary
This summary is machine-generated.

Near-infrared fluorescence imaging aids surgeons in visualizing tumors and metastases during surgery. This advanced technique enhances cancer detection and surgical precision but complements, rather than replaces, traditional diagnostic methods.

Keywords:
Cancer diagnosticFluorescent labelsFluorescent probesFluorophore-conjugated antibodiesIntraoperative fluorescence diagnosis

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Area of Science:

  • Medical Imaging
  • Oncology
  • Surgical Technology

Background:

  • Fluorescence imaging, particularly near-infrared (NIR) fluorescence, is advancing cancer detection and surgical guidance.
  • Current techniques utilize fluorescent agents and specialized cameras for enhanced visualization of tumors and surrounding tissues.

Purpose of the Study:

  • To review the latest advancements in fluorescence imaging technology for cancer applications.
  • To highlight the role of fluorescence-guided surgery (FGS) in improving surgical outcomes and detecting metastatic lesions.

Main Methods:

  • Review of current literature on NIR fluorescence imaging and FGS.
  • Analysis of the integration of fluorescence visualization in clinical practice for cancer detection.
  • Discussion of the development of targeted fluorescence imaging probes.

Main Results:

  • NIR fluorescence imaging combined with targeted tracers and cameras effectively visualizes tumors and aids in navigating surgical sites.
  • FGS assists surgeons in distinguishing anatomical structures, assessing tissue perfusion, and identifying metastases intraoperatively.
  • Clinical application shows significant potential for fluorescence visualization as an adjunct to standard cancer diagnostics.

Conclusions:

  • Fluorescence imaging is a valuable auxiliary tool in cancer surgery, improving visualization and precision.
  • It complements, but does not replace, established diagnostic methods like biopsy and CT scans.
  • Future developments focus on highly selective fluorescent probes for precise cancer cell targeting and delineation of tumor margins.