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Related Experiment Videos

Urinary organic acids: isolation and quantification for routine metabolic screening.

J Greter, C E Jacobson

    Clinical Chemistry
    |April 1, 1987
    PubMed
    Summary

    This study introduces a new method for isolating organic acids from urine using Porapak resins, achieving comparable or better recoveries than traditional methods. This technique aids in detecting metabolic disorders.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Metabolic Disorders

    Background:

    • Accurate isolation of organic acids from biological samples is crucial for diagnosing metabolic diseases.
    • Existing methods like solvent extraction and ion exchange have limitations in efficiency and recovery rates.

    Purpose of the Study:

    • To develop and evaluate a novel method for isolating organic acids from acidified urine.
    • To compare the efficacy of this new method with established techniques.

    Main Methods:

    • Utilized an equivolume mixture of Porapak Q and Porapak T for organic acid isolation from urine.
    • Compared results with ethyl acetate solvent extraction and DEAE-Sephadex ion-exchange chromatography.
    • Quantified separated acids using mass spectrometry and flame ionization detection.

    Main Results:

    • The Porapak method demonstrated equal or superior average recoveries for various 14C-labeled organic acids compared to solvent extraction.
    • Ion-exchange chromatography yielded higher recoveries for specific acids like oxalic, lactic, and citric acid.
    • Mass spectrometry and flame ionization detection showed good correlation for acid quantification.

    Conclusions:

    • The Porapak-based method offers an effective approach for organic acid isolation in routine metabolic screening.
    • This method has successfully identified patients with inborn errors of metabolism.
    • The findings support the utility of this technique in clinical diagnostics.

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