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An Efficient Strategy for Constructing Fluorescent Nanoprobes for Prolonged and Accurate Tumor Imaging
Yaling Wang1, Yong Zhang1, Mingrui Li1
1Center of Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry, Beijing University of Technology, 100124 Beijing, P. R. China.
Analytical Chemistry
|January 31, 2024
Summary
We developed activatable fluorescent nanoprobes using bioorthogonal reactions and gold-sulfur interactions for enhanced tumor imaging. This strategy improves probe retention and accuracy in detecting tumor biomarkers like alkaline phosphatase (ALP) in vivo.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Medical Imaging
Background:
- Activatable near-infrared (NIR) fluorescent probes offer high selectivity and sensitivity for tumor diagnosis.
- Small-molecule probes face limitations due to rapid diffusion and clearance in vivo.
- Developing nanoprobes with enhanced retention is crucial for accurate tumor imaging.
Purpose of the Study:
- To create a novel strategy for constructing activatable fluorescent nanoprobes.
- To achieve prolonged and high-contrast tumor imaging in vivo.
- To improve the diagnostic accuracy of tumor biomarkers.
Main Methods:
- Utilized bioorthogonal reactions and gold-sulfur (Au-S) interactions to construct nanoprobes.
- Developed an activatable nanoprobe (hCy-ALP@AuNP) for imaging alkaline phosphatase (ALP) activity.
- Leveraged the enhanced permeability and retention (EPR) effect for nanoparticle accumulation.
Main Results:
- The nanoprobe (hCy-ALP@AuNP) demonstrated nanoscale properties facilitating tumor accumulation and retention.
- Tumor-overexpressed ALP significantly enhanced the NIR fluorescence signal of the nanoprobe.
- Compared to small-molecule probes, hCy-ALP@AuNP showed improved tumor distribution, retention time, and imaging accuracy.
Conclusions:
- The developed nanoprobe platform enables efficient construction of biomarker-responsive fluorescent nanoprobes.
- This strategy holds significant potential for precise in vivo tumor diagnosis.
- Nanoprobe design overcomes limitations of small-molecule probes for improved tumor imaging.
