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Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

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Complete genome sequence of enterococcal phage G01.

Emma K Sheriff¹, Shelby E Andersen¹, Anushila Chatterjee¹

¹Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.

Microbiology Resource Announcements

|January 31, 2024

Summary

We sequenced the genome of enterococcal phage G01, finding it infects over 28% of tested Enterococcus faecalis strains. This phage does not require the enterococcal phage infection protein PIP_EF for infection.

Keywords:

Enterococcus bacteriophages genomics

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Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

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Published on: May 26, 2013

Area of Science:

Microbiology
Genomics
Virology

Background:

Enterococcus faecalis is an opportunistic pathogen.
Bacteriophages (phages) are viruses that infect bacteria and can be used as an alternative to antibiotics.
Understanding phage-host interactions is crucial for phage therapy development.

Purpose of the Study:

To report the annotated genome of enterococcal phage G01.
To determine the host range of phage G01 against Enterococcus faecalis strains.
To investigate the requirement of the enterococcal phage infection protein PIP_EF for G01 infection.

Main Methods:

Genome sequencing and annotation of enterococcal phage G01.
Host range analysis by plaque assays on Enterococcus faecalis isolates.
Comparative genomic analysis to identify potential infection mechanisms.

Main Results:

The G01 genome is 41,189 bp and contains 67 predicted open reading frames.
Phage G01 infected 28.6% (6/21) of the tested Enterococcus faecalis strains.
Phage G01 does not appear to require the enterococcal phage infection protein PIP_EF.

Conclusions:

The annotated genome of enterococcal phage G01 provides valuable genetic information.
Phage G01 exhibits a specific host range within Enterococcus faecalis.
The lack of PIP_EF requirement suggests alternative infection pathways for G01, expanding knowledge of phage-host interactions.