Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

GPI Anchoring of Proteins in the ER Membrane01:29

GPI Anchoring of Proteins in the ER Membrane

4.1K
GPI-anchoring is a post-translational, reversible protein modification that is ubiquitous in eukaryotes. Such proteins are primarily present on the exoplasmic leaflet of the plasma membrane.
GPI-anchor structure
A sequence of 11 enzymatic reactions results in the synthesis of the complete GPI anchor consisting of a hydrophobic and a hydrophilic portion. The hydrophobic portion comprises phosphatidylinositol, while the hydrophilic part comprises polar groups like phosphoethanolamine,...
4.1K
Lipids as Anchors01:32

Lipids as Anchors

5.6K
In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
5.6K
Anchoring Junctions01:03

Anchoring Junctions

3.8K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.8K
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

7.2K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
7.2K
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

12.1K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
12.1K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emotion Regulation and Psychological Adjustment in Residential Care: Relational and Gender Dynamics.

Journal of adolescence·2026
Same author

Oropharyngeal Colonization by Kingella kingae and Septic Arthritis in Children 6-48 Months of Age: A Portuguese Multicenter Case-control Study.

The Pediatric infectious disease journal·2026
Same author

Transradial neurointervention with the Benchmark BMX81 guide catheter: multicenter European experience.

Journal of neurointerventional surgery·2026
Same author

A moving white spot: What's the diagnosis?

Anales de pediatria·2026
Same author

Parental Attachment and Mental Health Literacy in Young Adults: The Mediational Role of Emotional Regulation on Well-Being and Psychopathological.

Scandinavian journal of psychology·2026
Same author

Agronomic Biofertilizer Potential of a Cyanobacterium-Enriched Culture.

Physiologia plantarum·2026

Related Experiment Video

Updated: Jul 4, 2025

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization
08:16

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

Published on: September 20, 2021

3.9K

JAGGER localization and function are dependent on GPI anchor addition.

Raquel Figueiredo1, Mónica Costa1,2, Diana Moreira1

  • 1LAQV/REQUIMTE, Departamento de Biologia, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre s/n, 4169-007, Porto, Portugal.

Plant Reproduction
|January 31, 2024
PubMed
Summary
This summary is machine-generated.

Correct glycosylphosphatidylinositol (GPI) anchor addition is crucial for JAGGER protein localization and function in Arabidopsis. Disrupting GPI anchor attachment in JAGGER impairs its cell periphery localization and ability to prevent polytuby, ensuring proper fertilization.

Keywords:
ArabidopsisArabinogalactan proteins (AGPs)GPI-anchored proteins (GPI-APs)Pollen–pistil interactionsPolytubey

More Related Videos

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
13:08

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published on: August 10, 2017

10.8K
Recording Gap Junction Current from Xenopus Oocytes
09:04

Recording Gap Junction Current from Xenopus Oocytes

Published on: January 21, 2022

2.2K

Related Experiment Videos

Last Updated: Jul 4, 2025

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization
08:16

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

Published on: September 20, 2021

3.9K
Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass
13:08

Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

Published on: August 10, 2017

10.8K
Recording Gap Junction Current from Xenopus Oocytes
09:04

Recording Gap Junction Current from Xenopus Oocytes

Published on: January 21, 2022

2.2K

Area of Science:

  • Plant reproductive biology
  • Molecular plant science
  • Cell biology

Background:

  • Successful double fertilization in flowering plants depends on precise sperm cell delivery.
  • Arabidopsis ovules prevent polyspermy (multiple pollen tube attraction) via the polytuby block.
  • JAGGER, a putative arabinogalactan protein, is essential for regulating pollen tube entry.

Purpose of the Study:

  • To investigate the role of glycosylphosphatidylinositol (GPI) anchor addition in JAGGER protein localization and function.
  • To determine if the GPI anchor is necessary for JAGGER's in vivo activity in preventing polytuby.

Main Methods:

  • Constructing and expressing JAGGER fused to citrine yellow fluorescent protein (JAGGER-cYFP) in Arabidopsis.
  • Creating JAGGER variants with deletions in predicted GPI anchor addition domains.
  • Analyzing JAGGER localization using fluorescence microscopy.
  • Assessing the ability of JAGGER variants to rescue the polytuby phenotype in jagger mutants.

Main Results:

  • Functional JAGGER-cYFP localized to the periphery of ovule integuments and transmitting tract cells.
  • Deletion of predicted GPI anchor addition domains disrupted JAGGER's peripheral localization.
  • JAGGER proteins with compromised GPI anchor addition failed to rescue the polytuby phenotype.

Conclusions:

  • GPI anchor addition is essential for JAGGER's correct localization to the cell periphery.
  • Proper JAGGER localization, dependent on GPI anchoring, is critical for its in vivo function in preventing polytuby and ensuring successful fertilization in Arabidopsis.