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Collection of Frozen Rodent Brain Regions for Downstream Analyses

Collection of Frozen Rodent Brain Regions for Downstream Analyses

Published on: April 23, 2020

Forensically relevant anatomical brain regions cannot be sub-differentiated by RNA expression analysis.

Jan Euteneuer¹, Lucas Moitinho-Silva², Cornelius Courts^3,4

¹Institute of Forensic Medicine, University Hospital of Schleswig-Holstein, Arnold-Heller-Strasse 12, 24105, Kiel, Germany.

Forensic Science, Medicine, and Pathology

|January 31, 2024

Summary

This summary is machine-generated.

Forensic RNA analysis of brain tissue is challenging. This study found mRNA expression differences between cerebral cortex regions are too subtle for reliable sub-differentiation in criminal investigations.

Keywords:

Brain regions Forensic genetics Head trauma Organ tissue identification RNA analysis RNA sequencing

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Area of Science:

Forensic science
Molecular biology
Neuroscience

Background:

Biological traces from head injuries can aid criminal investigations.
Differentiating specific brain regions from forensic samples is crucial for accurate analysis.

Purpose of the Study:

To identify and validate messenger RNA (mRNA) candidates for sub-differentiating forensically relevant brain regions.
To assess the feasibility of RNA expression analysis for forensic applications.

Main Methods:

Massively parallel sequencing of whole transcriptomes from frontal, temporal, parietal, and occipital lobes.
Bioinformatical analysis, classification, and biostatistical selection of mRNA candidates.
Validation using Multiplex-RT-PCR and capillary electrophoresis.

Main Results:

Minimal relative mRNA upregulation was observed, primarily in the parietal lobe.
Two candidates (PVALB, CDR2L) showed upregulation in the cerebellum; PVALB was not reliably detected, while CDR2L was present in all lobes.
Differences in mRNA expression across the four cerebral cortex regions were not pronounced enough for forensic application.

Conclusions:

Sub-differentiation of cerebral cortex regions using RNA expression analysis is generally not feasible for forensic purposes.
The subtle mRNA expression variations limit the utility of this approach in forensic investigations.