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Updated: Jul 4, 2025
Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
Published on: November 14, 2016
Protocol for near-infrared optogenetics manipulation of neurons and motor behavior in C. elegans using emissive
Ruipeng Wang1, Jingxuan Guo2, Hanlu Yao1
1College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Abstract:
In deep tissue, optogenetics faces limitations with visible light. Here, we present a protocol for near-infrared (NIR) optogenetics manipulation of neurons and motor behavior in Caenorhabditis elegans using emissive upconversion nanoparticles (UCNPs). We describe steps for synthesizing and modifying UCNPs. We then detail procedures for regulating neurons using these UCNPs in the model organism C. elegans. Using NIR light allows for superior tissue penetration to manipulate neuronal activities and locomotion behavior. For complete details on the use and execution of this protocol, please refer to Guo et al.,1 Ao et al.,2 and Zhang et al.3.
