Protocol for lentiviral vector-based gene transfection in human ILC2s
Misato Irie1, Hiroki Kabata1, Koichi Fukunaga1
1Keio University School of Medicine, Department of Medicine, Division of Pulmonary Medicine, Tokyo, Japan.
Modifying gene expression in lymphocytes is essential for immunological research; however, gene transfection methods for group 2 innate lymphoid cells (ILC2s) are not well established. Here, we present a protocol utilizing lentiviral vectors to introduce genes into human ILC2s. We detail steps for lentiviral solution preparation, transfection, and selection of transfected cells. This protocol allows overexpression or suppression of specific genes and evaluation of the changes in ILC2 biology. For complete details on the use and execution of this protocol, please refer to Irie et al. (2023).1.
Modifying gene expression in lymphocytes is essential for immunological research; however, gene transfection methods for group 2 innate lymphoid cells (ILC2s) are not well established. Here, we present a protocol utilizing lentiviral vectors to introduce genes into human ILC2s. We detail steps for lentiviral solution preparation, transfection, and selection of transfected cells. This protocol allows overexpression or suppression of specific genes and evaluation of the changes in ILC2 biology. For complete details on the use and execution of this protocol, please refer to Irie et al. (2023).1.
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