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In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

Published on: May 16, 2011

Minimum Information for Conducting and Reporting In Vitro Intracellular Infection Assays.

Santhni Subramaniam¹, Paul Joyce¹, Abiodun D Ogunniyi²

¹UniSA Clinical and Health Sciences, University of South Australia, Adelaide, SA 5000, Australia.

ACS Infectious Diseases

|January 31, 2024

Summary

New guidelines aim to improve reproducibility in antimicrobial research. These standards will help compare studies on novel therapeutics for intracellular bacterial infections, aiding clinical translation.

Keywords:

Guidelines cellular studies drug delivery infection assay intracellular infection nanoantibiotics

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Last Updated: Jul 27, 2026

In Vitro Assay of Bacterial Adhesion onto Mammalian Epithelial Cells

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Published on: May 16, 2011

Early Viral Entry Assays for the Identification and Evaluation of Antiviral Compounds

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Published on: October 29, 2015

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

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Published on: November 16, 2016

Area of Science:

Microbiology
Infectious Diseases
Drug Development

Background:

Bacterial pathogens develop resistance to host defenses and antibiotics.
Intracellular bacterial infections pose significant clinical challenges.
Novel strategies include intracellularly active molecules and nanoantibiotics.

Purpose of the Study:

To address inconsistencies and lack of reproducibility in research on intracellular bacterial infections.
To propose minimum information guidelines for publishing methodology and results.
To facilitate the translation of emerging antimicrobial technologies into clinical therapies.

Main Methods:

Development of guidelines divided into four categories: experimental design, in vitro model establishment, efficacy assessment, and statistical assessment.
Focus on essential parameters for researchers to disclose in publications.
Emphasis on improving rigor and enabling quantitative comparisons.

Main Results:

Guidelines are proposed to enhance the comparability and reproducibility of studies on intracellular infections.
The guidelines cover experimental design, in vitro models, efficacy testing, and statistical analysis.
Adherence to these guidelines is expected to improve the rigor of research.

Conclusions:

Implementing these minimum information guidelines will enhance scientific comparison and reproducibility.
Improved research rigor will facilitate the development of effective therapies for intracellular bacterial infections.
These guidelines aim to bridge the gap between pre-clinical research and clinical application.