Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

GWAS-informed genomic selection for cold tolerance in pepper (Capsicum annuum L.).

BMC plant biology·2026

Same author

Improved ecological risk assessment of phenol in sediments via species sensitivity distribution and equilibrium partitioning method using water toxicity data.

Scientific reports·2026

Same author

Establishment and Comparison of High-Throughput Immunoassays for Quantifying Clostridioides difficile Infection Biomarkers.

Biotechnology journal·2026

Same author

Development of a novel method for generating Q-bodies using tyrosinase-mediated HA-tag labeling.

Analytical and bioanalytical chemistry·2025

Same author

Establishment of a Rapid and Convenient Fluoroimmunoassay Platform Using Antibodies Against PDL1 and HER2.

Current issues in molecular biology·2025

Same author

Convenient production of a novel recombinant antibody against periodontitis biomarker S100A8.

Preparative biochemistry & biotechnology·2024

Search research articles

Related Experiment Video

Updated: Jul 4, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Fluorogenic enzyme-linked immunosorbent assay with a dual color variation.

Hanool Yun¹, Hee-Jin Jeong¹

¹Department of Biological and Chemical Engineering, Hongik University, 2639 Sejong-ro, Sejong-si 30016, South Korea.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy

|January 31, 2024

Summary

A new fluorogenic enzyme-linked immunosorbent assay (FELISA) enables sensitive detection of tumor necrosis factor alpha (TNFα) and programmed cell death-ligand 1 (PDL1). This high-throughput method offers rapid and accurate biomarker analysis for disease diagnosis and treatment.

Keywords:

Fluorescent biosensor Fluorogenic enzyme-linked immunosorbent assay Immunoassay Programmed cell death-ligand 1 Tumor necrosis factor alpha

More Related Videos

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

Related Experiment Videos

Last Updated: Jul 4, 2025

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Published on: December 12, 2011

Area of Science:

Biochemistry
Immunology
Assay Development

Background:

Accurate biomarker detection is vital for disease diagnosis, monitoring, and treatment.
Existing immunoassay tools may lack the required sensitivity or throughput for certain applications.

Purpose of the Study:

To develop a sensitive and high-throughput fluorogenic enzyme-linked immunosorbent assay (FELISA).
To detect key biomarkers: tumor necrosis factor alpha (TNFα) and programmed cell death-ligand 1 (PDL1).

Main Methods:

Development of a FELISA utilizing Amplex Red or QuantaBlu fluorescent substrates.
Application of the assay for the detection of TNFα and PDL1.

Main Results:

Achieved a limit of detection in the nanogram order for TNFα and PDL1.
Enabled convenient assaying of multiple samples within 20 hours.
Demonstrated FELISA's applicability as a powerful immunoassay tool.

Conclusions:

FELISA provides a rapid and accurate method for detecting TNFα and PDL1.
This assay platform is versatile and can be adapted for various fluorogenic immunoassays against other antigens.