Nanozyme-based Clusterphene for Enhanced Electrically Catalytic Cancer Therapy

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Catalysis

Background:

• Nanozyme-based catalytic therapy shows promise for cancer treatment.
• Nanozyme aggregation into 3D structures often impairs catalytic efficiency.
• Novel strategies are needed to enhance nanozyme performance in vivo.

Purpose of the Study:

• To design and investigate nanozyme-based clusterphenes for enhanced cancer catalytic therapy.
• To explore the synergistic effects of polyoxometalate (POM) and glucose oxidase (GOx) in a layered structure.
• To evaluate the impact of electric fields on the catalytic activity and therapeutic efficacy.

Main Methods:

• Assembly of polyoxometalate (POM) and glucose oxidase (GOx) into layered nanozyme clusterphenes with hexagonal pores.
• Investigation of multi-enzyme activities: peroxidase (POD)-like, catalase (CAT), and glutathione oxidase (GPx).
• Assessment of nanozyme performance under electrical stimulation using in vitro and in vivo tumor models.

Main Results:

• The POMG-based clusterphene exhibited significantly enhanced POD-like activity (sevenfold increase under electrical stimulation).
• Sustained production of reactive oxygen species (ROS) and consumption of tumor glucose by GOx.
• Demonstrated efficient tumor inhibition and prevention of proliferation in a murine model.

Conclusions:

• POMG-based clusterphenes offer a promising platform for enhanced nanozyme catalytic therapy.
• The layered structure and electrical stimulation synergistically boost therapeutic efficacy.
• This approach holds potential for future cancer treatment strategies.